Xu Chen, Jia-Ming Ma, Ke-Lan Yong, Jing-Ci Lv, Xia-Bing Zhang, "Fluorescence study on the interaction of human serum albumin with loureirin B", Journal of Spectroscopy, vol. 24, Article ID 893430, 11 pages, 2010. https://doi.org/10.3233/SPE-2010-0476
Fluorescence study on the interaction of human serum albumin with loureirin B
The interaction between loureirin B (Lour B) and human serum albumin (HSA) was investigated by fluorescence and UV–vis absorption spectroscopy. Experimental results indicated that loureirin B had a strong ability to quench the intrinsic fluorescence of HSA through a dynamic quenching procedure. The fluorescence quenching data revealed that the quenching constants (KSV) 2.68×104, 3.30×104 and 4.10×104 l/mol at 300, 310 and 320 K, respectively. Based on the thermodynamic parameters obtained, the positive values of enthalpy change ΔH and entropy change ΔS suggested that hydrophobic forces played a major role in the interaction of Lour B with HSA. According to Förster theory of energy transfer, the distance r between HSA and Lour B was calculated to be 2.85 nm. Furthermore, the effect of Lour B on the conformation of HSA was analyzed by synchronous fluorescence and three-dimensional fluorescence spectra.
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