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Volume 25 (2011), Issue 1, Pages 45-51

Prooxidative and fluorescence properties of paracetamol during interactions with mitochondria

Vladimíra Tomečková,1,2 Alena Gajová,1 Beáta Veliká,1 Lýdia Saxunová,1 and Zdenka Hertelyová1

1Department of Medical Chemistry, Biochemistry and Clinical Biochemistry, Faculty of Medicine UPJŠ, Košice, Slovakia
2Department of Medical Chemistry, Biochemistry and Clinical Biochemistry, Faculty of Medicine UPJŠ, Trieda SNP 1, Košice 040 66, Slovakia

Copyright © 2011 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


This study was carried out to investigate isolated liver mitochondrial functions after paracetamol administration by monitoring of liver mitochondrial fluorescence properties as well as prooxidative properties of paracetamol. Paracetamol was administered to rat (in subtoxic 500 mg·kg−1 dose) in vivo. The effect of this dose was compared with the subtoxic and toxic dose of paracetamol added to mitochondria in vitro (1 and 1.5) mg paracetamol/mg mitochondrial protein. Subtoxic dose of paracetamol in vitro did not change mitochondrial fluorescence, but it significantly decreased mitochondrial fluorescence in vivo in comparison with control mitochondrial group. Toxic dose of paracetamol in vitro significantly decreased mitochondrial fluorescence. The enzymatic activity of superoxide dismutase (SOD) significantly decreased after paracetamol administration in vitro and in vivo. While both activities of glutathione peroxidase (GPx) and glutathione reductase (GR) significantly increased in dependence upon paracetamol doses. Our experiment showed, that paracetamol participates in formation of free radicals and confirms previous studies, in which paracetamol administration caused elevation of antioxidative enzymes activities in dependence on dose, which is considered therapeutically as subtoxic and toxic.