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Volume 25, Issue 5, Pages 217-224

Characterization and discrimination of nasopharyngeal carcinoma and nasopharyngeal normal cell lines using confocal Raman microspectroscopy

Yuhuang Ye,1,2 Yang Chen,1,2 Yongzeng Li,1 Ying Su,3 Changyan Zou,3 Yanping Chen,3 Lin Ou,1 Rong Chen,1,5 and Haishan Zeng4

1Key Laboratory of Optoelectronic Science and Technology for Medicine, Ministry of Education, Fujian Normal University, Fuzhou, Fujian, China
2College of Physics and Information, Fuzhou University, Fuzhou, Fujian, China
3Fujian Provincial Tumor Hospital, Fuzhou, Fujian, China
4Cancer Imaging Department, British Columbia Cancer Research Centre, Vancouver, BC, Canada
5Key Laboratory of Optoelectronic Science and Technology for Medicine, Ministry of Education and Fujian Provincial Key Laboratory for Photonics Technology, Fujian Normal University, Fuzhou 350007, China

Copyright © 2011 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Raman microspectroscopy can provide molecular-level information about the biochemical composition and structure of cells and tissues with excellent spatial resolution. In this study, Raman spectroscopy of individual cells from nasopharyngeal carcinoma cell lines C666-1, CNE2 and nasopharyngeal normal cell line NP69 are investigated for their differences. The spectral intensity ratio at 1449 and 1657 cm−1 with a decision line of I1449/I1657=1.10 can very easily separate the tumor and normal cell lines into two groups. Principal component analysis (PCA) and linear discriminant analysis (LDA) are also used to classify different cell lines and achieved a specificity and sensitivity of 100 and 90%, respectively. The results support the potential utility of Raman spectroscopy for nasopharyngeal diagnosis.