Hamid Dezhampanah, Termeh Darvishzad, Mehrnaz Aghazadeh, "Thermodynamic and spectroscopic study on the binding of interaction anionic phthalocyanine with calf thymus DNA", Journal of Spectroscopy, vol. 26, Article ID 452328, 9 pages, 2011. https://doi.org/10.3233/SPE-2012-0564
Thermodynamic and spectroscopic study on the binding of interaction anionic phthalocyanine with calf thymus DNA
The interaction between anionic form of copper (II) N,N',N",N'"-tetrasulfonated phthalocyanine Cu (tspc) and to calf thymus deoxyribonucleic acid (ct-DNA) is investigated by measuring UV-vis absorption and fluorescence spectroscopy in phosphate buffer. The binding constant and stoichiometry were determined by analysis of optical absorption spectra of phthalocyanine at various ct-DNA concentrations using SQUAD software. The static mode of fluorescence quenching of phthalocyanine by calf thymus deoxyribonucleic acid indicates the formation of a ground-state complex. The formation of ground-state complex is a spontaneous molecular interaction procedure in which outside groove binding through the formation of an axial bond between the base pairs of nucleotide and Cu in the central core of phthalocyanine.
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