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Volume 26 (2011), Issue 2, Pages 129-139

Laser-induced chlorophyll fluorescence and reflectance spectroscopy of cadmium treated Triticum aestivum L. plants

Jitendra Kumar Pandey1 and R. Gopal1,2

1M.N. Saha Center of Space Studies, IIDS, Nehru Science Center, University of Allahabad, Allahabad, India
2Laser Spectroscopy and Nanomaterials Laboratory, Department of Physics, University of Allahabad, Allahabad, India

Copyright © 2011 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The present study deals with laser-induced chlorophyll fluorescence (LICF) spectra, reflectance spectra and fluorescence induction kinetics (FIK) curves of Triticum aestivum L. plants treated with different concentrations of cadmium (0.01, 0.1 and 1.0 mM). LICF spectra were recorded in the region of 650–780 nm using violet diode laser (405 nm) and FIK curves were recorded at 685 and 730 nm using red diode laser (635 nm) for excitation. Reflectance spectra were recorded in the region of 400–800 nm using spectrophotometer with an integrating sphere. The fluorescence intensity ratios (FIR) were determined from LICF spectra, vitality index (Rfd) from FIK curves and narrow band vegetation index (NBVI) from reflectance spectra. These parameters along with plant growth parameters and photosynthetic pigment contents were used to analyze the effect of cadmium on wheat plants. The results clearly show that lower concentration of Cd (0.01 mM) shows stimulatory response; whereas higher concentrations (0.1 and 1.0 Mm) are hazardous for plant growth, photosynthetic pigments and photosynthetic activity of wheat plants.