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Spectroscopy
Volume 26, Issue 3, Pages 187-194
http://dx.doi.org/10.3233/SPE-2011-0540

SERS spectra of a single nasopharyngeal carcinoma cell based on intracellularly grown and passive uptake Au nanoparticles

Hao Huang,1 Weiwei Chen,1,2 Jianji Pan,3 Qisong Chen,3 Shangyuan Feng,2,5 Yun Yu,1,2 Yanping Chen,4 Ying Su,3 and Rong Chen2

1College of Integrated Traditional Chinese and Western Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, China
2Key Laboratory of Optoelectronic Science and Technology for Medicine, Ministry of Education, Fujian Provincial Key Laboratory of Photonic Technology, Fujian Normal University, Fuzhou, China
3Fujian Provincial Cancer Hospital, Fuzhou, China
4Fujian Provincial Tumor Hospital, Fuzhou, China
5Key Laboratory of Optoelectronic Science and Technology for Medicine, Ministry of Education, Fujian Provincial Key Laboratory of Photonic Technology, Fujian Normal University, Fuzhou 350007, China

Copyright © 2011 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The intracellularly-grown-Au-nanoparticles (IGAuNs) technique was employed to analyze the surface-enhanced Raman scattering (SERS) spectra of nasopharyngeal carcinoma cells (CNE-1 cell line). There are only six obvious Raman bands (718, 1001, 1123, 1336, 1446, 1660 cm−1) in the normal Raman spectrum of living CNE-1 cells. However, over twenty SERS Raman bands have been detected in the SERS spectra of IGAuNs-induced cells, among which five bands are of the DNA backbone (673, 1097, 1306, 1336 and 1585 cm−1). There are four vibrations of the DNA backbone (1026, 1097, 1336 and 1585 cm−1) in the SERS spectra of living CNE-1 cells induced by the passive uptake gold nanoparticles (PUAuNS), but one more DNA backbone and many nucleus Raman peaks appeared in the IGAuNs-induced SERS spectra. Many Raman peaks in the PUAuNs-induced SERS spectra are stronger than those in the IGAuNs-induced ones. This study has shown that the PUAuNs technique can achieve stronger Raman signals, and that the IGAuNs technique can enable the gold element to access to the nucleus more easily, which could help to obtain more surface-enhanced Raman signals of the intracellular biochemical molecules. Thus, the two techniques can work together to attain the Raman spectral information of the cytoplasm and the nucleus in a better way, which might provide a sensitive method for broad biomedical applications such as intracellular SERS analysis of living cells.