Determination of Collagen Fibers Arrangement in Bone Tissue by Using Transformations of Raman Spectra Maps

Figure 2

The contrast images based on the ratios of 𝜈₂PO₄₃₋/amide III ((a), (b), and (g)), 𝜈₁CO₃₂₋/𝜈₂PO₄₃₋ ((b), (e), and (h)), and 𝜈₁PO₄₃₋/amide I ((c), (f), (i)). Arrows indicate polarization of laser incident and scattered beams, and the color bar displays the maximum ratio for each image.