The Application of Biophysical Techniques to Study Antimicrobial Peptides
Figure 1
The use of spectroscopic methodologies to evaluate the interaction of AMPs with model membranes. (a) Partition of the AMP LEAP-2 into lipid vesicles followed by Trp fluorescence. An increase in the LEAP-2 fluorescence intensity upon titration with lipid vesicles is evident. The value can be determined applying (4.2). (b) Normalized fluorescence emission spectra of LEAP-2 illustrate a blue-shift upon titration with lipid vesicles. (c) Circular dichroism spectra of pardaxin 4 in the absence (full line) or presence of lipopolysaccharide (dashed line). Panels (a) and (b) were adapted from [7] and panel (c) was adapted from [8].
