Role of Vibrational Spectroscopy in Stem Cell Research

Figure 5

(A) Average Raman spectra of murine embryonic stem cells (mES) at various stages of differentiation: (a) undifferentiated, (b) 4 days differentiation, and (c) 16 days differentiation via the formation of embryoid bodies. Spectra are shifted vertically for clarity. (B) Area of Raman bands of nucleic acids (RNA 813 cm⁻¹ and DNA 788 cm⁻¹) in undifferentiated, 4 days differentiation, and 16 days differentiation via the formation of embryoid bodies (^∗𝑃>0.01,^∗∗𝑃<0.01 compared to mES cells).

(a)

(b)