Role of Vibrational Spectroscopy in Stem Cell Research

Figure 6

(a) Mean Raman spectra obtained from human BM-MSCs (red; 𝑛=122) and fibroblasts (blue; 𝑛=109) displayed in the fingerprint range from 600 cm⁻¹ to 1800 cm⁻¹. All data are detected as relative intensities in arbitrary units (a.u.). The difference spectrum (black line) is obtained by subtracting the spectrum of human BM-MSCs from the spectrum of fibroblasts. Loading values (grey line) are plotted for the first principal component (PC1), defined by the PCA plot. (b) The PCA dot plot shows two clearly distinguishable cell populations depicted as red (BM-MSCs) and blue (fibroblasts) clusters.

(a)

(b)