|
| Wavenumber (cm−1) | Assignment |
|
| 3328 | Amide A: N–H and O–H stretching vibrations of polysaccharides, proteins |
| 3129 | Amide B: N–H stretching vibrations of proteins |
| 3015 | Olefinic =CH stretching: unsaturated lipids, cholesterol esters |
| 2960 | CH3 antisymmetric stretching: lipids, protein side chains |
| 2920 | CH2 antisymmetric stretching: mainly lipids |
| 2875 | CH3 symmetric stretching: protein side chains, lipids |
| 2850 | CH2 symmetric stretching: mainly lipids |
| 1720–1745 | C=O stretching vibrations of lipids (triglycerides and cholesterol esters) |
| 1710–1716 | C=O antisymmetric stretching: RNA and purine base |
| 1705–1690 | C=O antisymmetric stretching vibrations: RNA, DNA |
| 1654 | Amide I: C=O (80%) and C–N (10%) stretching, N–H (10%) bending vibrations: proteins a-helix |
| 1630–1640 | Amide I: C=O (80%) and C–N (10%) stretching, N–H (10%) bending vibrations:proteins β-structure |
| 1610, 1578 | C4-C5 and C=N stretching in imidazole ring of DNA, RNA |
| 1515 | Aromatic tyrosine ring |
| 1540–1550 | Amide II: N–H (60%) bending and C–N (40%) stretching vibrations: proteins α-helix |
| 1530 | Amide II: N–H (60%) bending and C–N (40%) stretching vibrations: proteins β-structure |
| 1467 | CH2 bending vibrations: lipids and proteins |
| 1455 | CH3 bending and CH2 scissoring vibrations: lipids and proteins |
| 1370–1400 | COO– symmetric stretching and CH3 bending vibrations: lipids, proteins |
| 1330–1200 | Amide III: proteins |
| 1230–1244 | antisymmetric stretching vibrations: RNA, DNA and phospholipids |
| 1090-1080 | symmetric stretching vibrations: RNA, DNA |
| 1060, 1050 | C–O stretching vibrations: deoxyribose/ribose DNA, RNA |
| 996 | RNA stretch and bend ring of uracil |
| 965 | symmetric stretch (DNA) and deoxyribose-phosphate skeletal motions |
| 925–929 | Sugar vibrations in backbone of DNA-Z form |
|
