Fluorescence Lifetime Imaging of Propranolol Uptake in Living Glial C6 Cells

Table 1

Spectroscopic properties of tryptophan, serotonin, and propranolol including fluorescence quantum yields (𝜙_𝑓), fluorescence lifetimes (𝜏_𝑓), and 2-photon excitation cross sections (𝜎₂).

	Propranolol	Serotonin	Tryptophan
𝜀(295nm)^a	5310	4280	1490
𝜀(315nm)^a	1730	1140	20
𝜙𝑓	0.50±0.03	0.28 [20]	0.27 [21]
𝜏𝑓(ns)	9.8	3.9 [20]	2.79, 0.26 [21]
𝜎2(590nm)^b	0.40	0.94	0.21 [22]
𝜎2(630nm)^b	0.17^c,0.22^d	0.35	—

^aUnits M⁻¹ cm⁻¹, ^bGM units (10⁻⁵⁰ cm⁴ s photon⁻¹), ^cdetermined using tryptophan as standard, ^ddetermined with 9-chloroanthracene as standard.