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Spectroscopy: An International Journal
Volume 27 (2012), Article ID 530791, 7 pages

Background Estimation of Biomedical Raman Spectra Using a Geometric Approach

1Department of Medical Physics, Medical School, University of Ioannina, Ioannina 45110, Greece
2Department of Physics, University of Ioannina, Ioannina 45110, Greece

Copyright © 2012 Nikolaos Kourkoumelis et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Raman spectroscopy grows into an essential tool for biomedical applications. Nevertheless, the weak Raman signal associated mainly with biological samples is often obscured by a broad background signal due to the intrinsic fluorescence of the organic molecules present, making further analysis unfeasible. A computational geometry method based on the definition of convex hull is described to estimate the background from Raman spectra of samples with biological interest. The method is semiautomated requiring sample-dependent user intervention. It does not depend, however, on curve fitting, requires no information about background distribution or source, and keeps the original spectral data intact.