A Comparison between Vanadyl, Vanadate, and Decavanadate Effects in Actin Structure and Function: Combination of Several Spectroscopic Studies
Figure 1
Frozen aqueous solution X-band EPR spectra. (A) 2 mM Tris (pH 7.5), 0.2 mM CaCl2 and 25 mM decavanadate with (B) 100 μM G-actin, (C) 50 μM G-actin plus 0.2 mM ATP, (D) 2 mM Tris (pH 7.5), 0.2 mM CaCl2, and 250 μM VOSO4 with 50 μM G-actin, (E) same as (D) plus 0.2 mM ATP. Vanadium (IV) EPR typical signals were observed upon both decavanadate (B) or VOSO4 (D) incubation with G-actin. No EPR signals were detected when ATP was present in the medium (C and E).
