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Study on the interaction of Prodigiosin with bovine serum albumin by spectroscopic methods
Shu-Chao Liu,1,2,3 Jing Tang,4 Xi-Hai Zhang,1,2,3 Yuan-Yuan Gao,1,2,3 Fei Ma,4 and Qi-yin Yang
1,2,3,51College of Life Science, Nanjing Normal University, Nanjing, China
2Jiangsu Key Laboratory for Microbes and Functional Genomics, College of Life Sciences, Nanjing Normal University, Nanjing, China
3Jiangsu Province Engineering and Technology Research Center for Microbiology, College of Life Science, Nanjing Normal University, Nanjing, China
4Center for Analysis and Testing, Nanjing Normal University, Nanjing, China
5Jiangsu Province Engineering and Technology Research Center for Microbiology, College of Life Science, Nanjing Normal University, Nanjing 210046, China
Abstract
The interaction between bovine serum albumin (BSA) and Prodigiosin (PG) was investigated by UV-vis absorption, fluorescence, synchronous fluorescence, FT-IR and circular dichroism (CD) techniques. The data of UV-vis absorption and fluorescence spectra displayed that there existed interaction between PG and aromatic amino acid residues of BSA. The synchronous fluorescence and CD spectrum experiment both showed that the secondary structure of BSA changed with addition of PG. All these results revealed that the conformation and microenvironment of BSA were changed.
Copyright
Copyright © 2012 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
