Spectrofluorometric Determination of Certain Antihyperlipidemic Agents in Bulk and Pharmaceutical Preparations

Table 1

Results obtained by the proposed spectrofluorimetric method for the determination of the cited drugs.

Item	RSV	EZE	PIT	Binary Mixture RSV-EZE
				RSV	EZE
Λ ex of measurements	315 nm	260 nm	245 nm	260 nm	260 nm
Λ em of measurements	362 nm	309 nm	373 nm	309 nm	432 nm
LOD (μg mL^−1)	0.16	0.08	0.03	0.13	0.06
LOQ (μg mL^−1)	0.50	0.25	0.10	0.40	0.20
Range of linearity	0.50–10.00 μg⋅mL^−1	0.25–4.00 μg⋅mL^−1	0.10–3.00 μg⋅mL^−1	0.40–10.00 μg⋅mL^−1	0.20–5.00 μg⋅mL^−1
Regression equation	𝑦=72.4548𝑥+35.2794	𝑦=209.0371𝑥+17.9884	𝑦=278.9371𝑥+37.1792	𝑦=63.2419𝑥−1.3854	𝑦=185.9146𝑥+15.5349
Correlation coefficient (𝑟)	0.9997	0.9981	0.9999	0.9992	0.9986
Sb	0.5359	4.5194	1.2935	0.6601	2.8866
Sa	2.8323	9.2942	1.8911	3.6756	8.4757
Standard error of the estimation	5.0089	14.5280	3.4162	6.7294	14.5076
Intraday (RSD%)	0.39–0.98	0.01–0.08	0.02–0.48	0.22–0.68	0.03–0.24
Interday (RSD%)	0.32–0.80	0.26–0.74	0.06–0.33	0.23–0.42	0.12–0.24
Drug in bulk %	100.30±0.91	100.24±1.33	99.63±1.02	—	—
Drug in laboratory Prepared binary Mixture (RSV-EZE)%	—	—	—	100.06±1.44	100.46±1.25
Drug in dosage form (alone)%	101.67±1.14	102.52±0.93	98.94±0.73	—	—
Drug in dosage form (binary mixtures)%	—	—	—	100.29±1.41	107.07±1.31
Drug added (alone)%	100.12±1.53	100.26±1.04	99.82±1.36	—	—
Drug added (binary mixture)%	—	—	—	100.30±1.05	100.21±1.23

S_a: standard deviation of intercept, S_b: standard deviation of slope.