Table 1: Results obtained by the proposed spectrofluorimetric method for the determination of the cited drugs.

Item RSV EZE PIT Binary Mixture RSV-EZE
RSVEZE

Λ ex of measurements315 nm260 nm245 nm260 nm260 nm
Λ em of measurements362 nm309 nm373 nm309 nm432 nm
LOD (μg mL−1)0.160.080.030.130.06
LOQ (μg mL−1)0.500.250.100.400.20
Range of linearity0.50–10.00 μg mL−10.25–4.00 μg mL−10.10–3.00 μg mL−10.40–10.00 μg mL−10.20–5.00 μg mL−1
Regression equation 𝑦 = 7 2 . 4 5 4 8 𝑥 + 3 5 . 2 7 9 4 𝑦 = 2 0 9 . 0 3 7 1 𝑥 + 1 7 . 9 8 8 4 𝑦 = 2 7 8 . 9 3 7 1 𝑥 + 3 7 . 1 7 9 2 𝑦 = 6 3 . 2 4 1 9 𝑥 1 . 3 8 5 4 𝑦 = 1 8 5 . 9 1 4 6 𝑥 + 1 5 . 5 3 4 9
Correlation coefficient ( 𝑟 )0.99970.99810.99990.99920.9986
Sb0.53594.51941.29350.66012.8866
Sa2.83239.29421.89113.67568.4757
Standard error of the estimation5.008914.52803.41626.729414.5076
Intraday (RSD%)0.39–0.980.01–0.080.02–0.480.22–0.680.03–0.24
Interday (RSD%)0.32–0.800.26–0.740.06–0.330.23–0.420.12–0.24
Drug in bulk % 1 0 0 . 3 0 ± 0 . 9 1 1 0 0 . 2 4 ± 1 . 3 3 9 9 . 6 3 ± 1 . 0 2
Drug in laboratory Prepared binary Mixture (RSV-EZE)% 1 0 0 . 0 6 ± 1 . 4 4 1 0 0 . 4 6 ± 1 . 2 5
Drug in dosage form (alone)% 1 0 1 . 6 7 ± 1 . 1 4 1 0 2 . 5 2 ± 0 . 9 3 9 8 . 9 4 ± 0 . 7 3
Drug in dosage form (binary mixtures)% 1 0 0 . 2 9 ± 1 . 4 1 1 0 7 . 0 7 ± 1 . 3 1
Drug added (alone)% 1 0 0 . 1 2 ± 1 . 5 3 1 0 0 . 2 6 ± 1 . 0 4 9 9 . 8 2 ± 1 . 3 6
Drug added (binary mixture)% 1 0 0 . 3 0 ± 1 . 0 5 1 0 0 . 2 1 ± 1 . 2 3

Sa: standard deviation of intercept, Sb: standard deviation of slope.