Journal of Spectroscopy

Chlorophyll Fluorescence Spectra as an Indicator of X-Ray + EMS-Induced Phytotoxicity in Safflower

Table 1

Photosynthetic pigment contents and pigment ratios of control and X-ray + EMS-treated safflower plants.
X-ray + EMS treatmentChl a (μg/mL)Chl b (μg/mL)Total Chl (μg/mL)Chl a/b Car (μg/mL)Chl/Car
Control8.25 ± 0.061.92 ± 0.07 1 0 . 1 7 ± 0 . 0 6 4 . 2 9 ± 0 . 1 2 0 . 7 7 ± 0 . 1 2 1 3 . 1 4 ± 0 . 1 2
5 Kr + 0.5% EMS8.86 ± 0.05 (7.39)2.14 ± 0.04 (11.46) 𝟏 𝟏 . 𝟎 𝟎 ± 𝟎 . 𝟎 𝟓 (8.16) 𝟒 . 𝟏 𝟒 ± 𝟎 . 𝟎 𝟕 (–3.49) 𝟎 . 𝟕 𝟖 ± 𝟎 . 𝟎 𝟕 (1.30) 𝟏 𝟒 . 𝟏 𝟎 ± 𝟎 . 𝟎 𝟖 (7.31)
8 Kr + 0.5% EMS8.21 ± 0.06 (–0.48)1.76 ± 0.09 (–8.33) 9 . 9 7 ± 0 . 0 8 (–1.97) 4 . 6 6 ± 0 . 1 4 (8.62) 1 . 0 7 ± 0 . 0 9 (38.96) 9 . 3 0 ± 0 . 0 8 (–29.22)
12 Kr + 0.5% EMS7.64 ± 0.08 (–7.39)1.71 ± 0.07 (–10.94) 9 . 3 5 ± 0 . 0 8 (–8.06) 4 . 4 8 ± 0 . 1 3 (4.43) 0 . 9 2 ± 0 . 0 7 (19.48) 1 0 . 2 1 ± 0 . 1 6 (–22.30)
25 Kr + 0.5% EMS6.69 ± 0.12 (–18.91)1.22 ± 0.10 (–36.46) 7 . 9 1 ± 0 . 1 1 (–22.22) 5 . 4 7 ± 0 . 1 1 (27.51) 1 . 1 5 ± 0 . 2 4 (49.35) 6 . 8 8 ± 0 . 1 5 (–47.64)
± Values indicate standard deviation (mean n = 3). The values in parenthesis show percent decrease/increase over control plant.