Environmental Method to Determine Dopamine and Ascorbic Acid Simultaneously via Derivative Spectrophotometry

Table 1

Statistical analysis of the determination of dopamine and ascorbic acid in mixtures by first and second derivative spectrophotometry.

Compound	Order of derivative	Wavelength (nm)	Linearity range (mg L^−1)	Intercept	Slope	Regression coefficient	RSD (%)	Recovery (%)^a
DA	𝐷1	265.12	0.375–9.45	−0.016	78210.57	0.998	0.60	110.0
		251	0.375–9.45	1.00𝐸−4	1274.71	0.999	2.0	100.0
	𝐷2	281	0.375–9.45	−8.03𝐸−4	−1323.47	0.998	2.4	100.0
		259	0.352–3.52	−0.233	233212.29	0.999	1.22	100.0
	𝐷1	287	0.352–5.28	0.037	−282224.66	0.995	1.12	110.0
	𝐷2	275	0.352–5.28	6.12𝐸−4	−2062.77	0.997	2.2	101.0
AA		297	0.352–5.28	−4.03𝐸−4	1519.82	0.998	1.8	100.0

aSample: 3.78 mg L⁻¹ of DA and 3.52 mg L⁻¹ of AA.