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Journal of Spectroscopy
Volume 2013 (2013), Article ID 276981, 8 pages
Research Article

2,7-Dichlorofluorescein Hydrazide as a New Fluorescent Probe for Mercury Quantification: Application to Industrial Effluents and Polluted Water Samples

1Department of Studies in Chemistry, Bangalore University, Central College Campus, Bangalore 560 001, India
2Department of Microbiology & Biotechnology, Bangalore University, Jnanabharathi Campus, Bangalore 560 056, India

Received 19 June 2012; Accepted 19 September 2012

Academic Editor: Nives Galić

Copyright © 2013 Sureshkumar Kempahanumakkagari et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A new fluorescent probe 2,7-dichlorofluorescein hydrazide for mercury quantification in aqueous medium has been described. It is based on the spirolactam ring opening of colorless and nonfluorescent 2,7-dichlorofluorescein hydrazide induced by Hg2+ ions through the hydrolytic cleavage of amide bond to produce green-colored highly fluorescent dichlorofluorescein in alkaline medium. The significant color change of this reagent in the presence of mercury ions can be used as a sensitive naked-eye detector. The working range, limit of detection, and relative standard deviations were found to be 0.2–20 ngmL−1, 0.042 ngmL−1, and 0.69% respectively. The proposed method is free from most of the common interfering ions present in the environmental samples. The developed method has been successfully applied to determine trace level mercury from water, soil, and industrial effluents.