Site-Directed Mutagenesis of Myoglobin for Studies of Their Interaction with Iron(III) by Multi-Spectroscopic Techniques

Figure 1

The effect of Fe(III) on UV absorption spectra of Mb (WT), Mb (D44K), Mb (D60K), and Mb (K56D) (298 K) 𝐶_Mb = 5 × 10⁻⁶ mol·L⁻¹, 1–9. C (Fe(III))/10⁻⁶ mol·L⁻¹ = 0, 1, 2, 3, 4, 5, 6, 7, 8, 𝐶_(Mb(D44K)), 𝐶_(Mb(D60K)), 𝐶_(Mb(K56D)) = 5 × 10⁻⁶ mol·L⁻¹, C (Fe(III))/10⁻⁶ mol·L⁻¹ = 0, 0.5, 1, 1.5.

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