Site-Directed Mutagenesis of Myoglobin for Studies of Their Interaction with Iron(III) by Multi-Spectroscopic Techniques

Figure 2

Effect of Fe(III) on the fluorescence spectra of Mbs (25°C). C (Mb (WT, D44K, D60K): 5 × 10⁻⁶ mol·L⁻¹, 1–9, C (Fe(III))/10⁻⁵ mol·L⁻¹: 0, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, and 4, resp. C (Mb (K56D)): 5 × 10⁻⁶ mol·L⁻¹; 1–9, C (Fe(III))/10⁻⁶ mol·L⁻¹: 0, 1, 2, 3, 4, 5, 6, 7, 8, and 9, respectively.

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