Site-Directed Mutagenesis of Myoglobin for Studies of Their Interaction with Iron(III) by Multi-Spectroscopic Techniques

Table 1

The bimolecular quenching constant (𝐾_𝑞), the dynamic quenching constants (𝐾_𝑠𝑣), the correlation coefficients (𝑅) in Stern-Volmer curve.

Mb and Mutants	𝑇/K	𝐾𝑞/L⋅mol^−1⋅s^−1	𝐾𝑠𝑣/L⋅mol^−1
Mb (WT)	289	1.12×10^12	1.12×10^4
	298	1.04×10^12	1.04×10^4
	310	0.903×10^12	0.903×10^4
Mb (D44K)	289	1.51×10^12	1.51×10^4
	298	1.40×10^12	1.40×10^4
	310	1.29×10^12	1.29×10^4
Mb (D60K)	289	1.14×10^12	1.14×10^4
	298	1.09×10^12	1.09×10^4
	310	1.04×10^12	1.04×10^4
Mb (K56D)	289	1.7×10^12	1.7×10^4
	298	1.62×10^12	1.62×10^4
	310	1.46×10^12	1.46×10^4