Site-Directed Mutagenesis of Myoglobin for Studies of Their Interaction with Iron(III) by Multi-Spectroscopic Techniques

Table 2

The binding constants (𝐾_𝑎) and relative coefficient (𝑅) in lineweaver-Burk curve.

Mb and mutants	𝑇/K	𝐾𝑎/ L·mol^−1
Mb (WT)	289	1.06×10^4
	298	1.00×10^4
	310	0.926×10^4
Mb (D44K)	289	1.39×10^4
	298	1.23×10^4
	310	1.10×10^4
Mb (D60K)	289	1.18×10^4
	298	1.11×10^4
	310	1.03×10^4
Mb (K56D)	289	1.67×10^4
	298	1.61×10^4
	310	1.56×10^4