Site-Directed Mutagenesis of Myoglobin for Studies of Their Interaction with Iron(III) by Multi-Spectroscopic Techniques

Table 3

The binding constants (𝐾), the binding numbers (𝑛), and their correlations (𝑅) at different temperatures.

Mb mutants	𝑇/K	𝐾/( L·mol^−1)	𝑛	𝑅
Mb (WT)	289	1.48×10^4	1.0226	0.9997
	298	1.18×10^4	1.0128	0.9995
	310	9.81×10^4	1.0009	0.9994
Mb (D44K)	289	2.23×10^4	1.0424	0.9989
	298	1.78×10^4	1.0296	0.9986
	310	1.58×10^4	1.0282	0.9990
Mb (D60K)	289	1.47×10^4	1.0180	0.9989
	298	1.28×10^4	1.0135	0.9986
	310	1.12×10^4	0.9994	0.9990
Mb (K56D)	289	1.99×10^4	1.0133	0.999
	298	1.6×10^4	0.9989	0.999
	310	0.9×10^4	0.956	1