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Journal of Spectroscopy
Volume 2013, Article ID 685698, 4 pages
Research Article

Comparative Study on Molecular Weight of Konjac Glucomannan by Gel Permeation Chromatography-Laser Light Scattering-Refractive Index and Laser Light-Scattering Methods

1Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei University of Technology, Wuhan 430068, China
2Research Center of Food Fermentation Engineering and Technology of Hubei, Wuhan 430068, China
3College of Food Science & Technology, Huazhong Agricultural University, Wuhan 430070, China

Received 22 June 2012; Revised 24 August 2012; Accepted 29 August 2012

Academic Editor: Jisheng Pan

Copyright © 2013 Mei Xu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The gel permeation chromatography, combined with laser light scattering and refractive index (GPC-LLS-RI) online method and laser light scattering (LLS) single method, researched the weight-average molecular weight (Mw) of konjac glucomannan (KGM) in this paper. The results show that the determination results of molecular weight of KGM are similar by two method, the molecular weight of KGM is  g/mol by online method measured, and it is  g/mol by single method measured, compared with only 1.3% difference between the two methods. At the same time, the molecular weight distributions of KGM and conformation index were analyzed by online method, while the second virial coefficient () that characterizes the interaction between KGM molecules and solvent molecules was acquired by single method, which can provide reliable foundation for the molecular chain morphology research of KGM.