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Journal of Spectroscopy
Volume 2013, Article ID 903450, 6 pages
Research Article

Raman Spectroscopic Analysis of H2O2-Stimulated Three-Dimensional Human Skin Models Containing Asian, Black, and Caucasian Melanocytes

1Cellular Informatics Laboratory, Advanced Science Institute, RIKEN, Wako 351-0198, Japan
2Department of Chemistry, School of Science and Technology, Kwansei-Gakuin University, Sanda 669-1337, Japan
3Kobe Technical Center, Procter and Gamble Japan, Kobe 658-0032, Japan
4Procter & Gamble International Operations S.A., Singapore Branch, Immunos No. 02-12, 8A, Biomedical Grove, Singapore

Received 11 September 2012; Accepted 17 November 2012

Academic Editor: Kong-Thon Tsen

Copyright © 2013 Shin-ichi Morita et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Reactive oxygen species generated in dermal cells of human skin is related to skin disorders or diseases. In this study, Raman analysis effectively clarified the identities of three types of human skin models after the models were stimulated with hydrogen peroxide. With the Caucasian skin model, the major Raman bands underwent large intensity changes within 4-5 days of stimulation. With the Black skin model, the Raman bands remained almost unchanged. The changes in the Asian skin model were unique compared to those in the above two. Eumelanin and pheomelanin are probably the main compounds that differentiate dermal cells in terms of their sensitivity to hydrogen peroxide.