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Journal of Spectroscopy
Volume 2013, Article ID 919180, 8 pages
Research Article

Development and Validation of Spectrophotometric Methods for the Determination of Rasagiline in Pharmaceutical Preparations

1Bezmialem Vakif University, Faculty of Pharmacy, Department of Analytical Chemistry, Fatih, 34093 Istanbul, Turkey
2Istanbul University, Faculty of Pharmacy, Department of Analytical Chemistry, Beyazit, 34116 Istanbul, Turkey
3Bezmialem Vakif University, Faculty of Pharmacy, Department of Pharmaceutical Biotechnology, Fatih, 34093 Istanbul, Turkey

Received 29 May 2013; Revised 16 July 2013; Accepted 18 July 2013

Academic Editor: Naoki Asakawa

Copyright © 2013 Serife Evrim Kepekci Tekkeli et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


This study presents three simple, rapid, and accurate spectrophotometric methods for the determination of Rasagiline (RSG) in pharmaceutical preparations. The determination procedures depend on the reaction of RSG with chloranilic acid for method A, tetrachloro-1,4-benzoquinone for method B, and 7,7,8,8-tetracyanoquinodimethane for method C. The colored products were quantitated spectrophotometrically at 524, 535, and 843 nm for methods A, B, and C, respectively. Different variables affecting the reaction were optimized. Linearity ranges of the methods with good correlation coefficients (0.9988–0.9996) were observed as 25–300 µg mL−1, 25–350 µg mL−1, and 50–500 µg mL−1 for methods A, B, and C, respectively. The formation of products takes place through different mechanisms. The sites of interaction were confirmed by elemental analysis using IR and 1H-NMR spectroscopy. The validation of the methods was carried out in terms of specificity, linearity, accuracy, precision, robustness, limit of detection, and limit of quantitation. No interference was observed from concomitants usually present in dosage forms. The methods were applied successfully to the determination of RSG in pharmaceutical preparations.