Simple Spectrophotometric Methods for Determination of Tenofovir Fumarate and Emtricitabine in Bulk Powder and in Tablets

Table 1

Validation data for the determination of TEN and EMT using the proposed spectrophotometric methods.

Item	TEN; (^1DR)	EMT; (^1D)
Concentration range (𝜇g mL^−1)	0.5–40	0.5–40
Correlation coefficient (𝑟)	0.99999	0.99996
Intercept (𝑎)	2.0×10^−4	−3.0×10^−5
%𝑦-intercept	0.242	−0.194
Slope (𝑏)	2.8×10^−4	9.0×10^−4
RSD% of slope	0.187	0.372
𝑆𝑎	9.0×10^−5	5.0×10^−5
𝑆𝑏	5.0×10^−5	3.0×10^−5
𝑆𝑦/𝑥	2.0×10^−4	1.0×10^−4
LOD (𝜇g mL^−1)	0.010	0.167
LOQ (𝜇g mL^−1)	0.031	0.556