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Journal of Spectroscopy
Volume 2014 (2014), Article ID 412039, 7 pages
Research Article

Quantitative Analysis of Dihydroxybenzenes in Complex Water Samples Using Excitation-Emission Matrix Fluorescence Spectroscopy and Second-Order Calibration

1Key Lab of Process Analysis and Control of Sichuan Universities, Yibin University, Yibin, Sichuan 644000, China
2Hospital, Yibin University, Yibin, Sichuan 644000, China
3The First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China
4Computational Physics Key Laboratory of Sichuan Province, Yibin University, Yibin 644007, China

Received 24 July 2014; Revised 21 September 2014; Accepted 22 September 2014; Published 13 October 2014

Academic Editor: Lu Yang

Copyright © 2014 Hui Chen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The dihydroxybenzenes are organic intermediates in many fields for various purposes and have been widely recognized as fatal environmental pollutants. Simultaneous determination of these compounds is particularly important. These habitual methods are time-consuming and laborious. The combination of two-dimensional excitation-emission matrix (EEM) fluorescence and second-order calibration of parallel factor analysis (PARAFAC) was investigated for simultaneously determining catechol, hydroquinone, and tryptophan. A total of 25 samples were designed and are divided into a calibration set and a test set. An unexpected constituent was used as unknown interference. The EEM data were successfully decomposed into a four-factor model of PARAFAC. The resolved spectra excitation and emission profiles from PARAFAC algorithm were compared with the corresponding pure spectra to confirm the compounds in samples. Based on the decomposition, the final calibration models provided satisfactory concentration estimates. The mean recovery percentages were 98.3%, 101.7%, and 97.9% for catechol, hydroquinone, and tryptophan, respectively. The results reveal that the developed method is maybe a potential tool for simultaneous determination of phenolic components in water samples or other complex samples.