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Journal of Spectroscopy
Volume 2014 (2014), Article ID 517089, 5 pages
http://dx.doi.org/10.1155/2014/517089
Research Article

Liquid Chromatography-Tandem Mass Spectrometric Assay for Determination of Stavudine in Human Plasma

Key Laboratory of Applied Chemistry, Yanshan University, Qinhuangdao, Hebei 066004, China

Received 10 March 2014; Revised 9 May 2014; Accepted 21 May 2014; Published 17 June 2014

Academic Editor: Bing Wu

Copyright © 2014 Fengdan Jin. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A LC-MS/MS method for determination of stavudine in human plasma was established and validated, and it was applied to the pharmaceutical formulations bioequivalence study. 0.5 mL plasma sample was extracted by liquid-liquid extraction. Stavudine was detected by a LC-MS/MS system. The pharmacokinetic parameters of stavudine in different formulations were calculated by noncompartment model statistics. The method was linear over the concentration ranges 5.00–1000 ng/mL in plasma. The intra- and interassay relative standard deviation (RSD) was <10%. The average accuracies for the assay at three concentrations (5.00, 80.0, and 900 ng/mL) were from 100.2% to 102.5%. Pharmacokinetic parameters of stavudine reference formulation were obtained as follows: was  h, was  g/L, t1/2 was  h, and was  g·h/L, and pharmacokinetic parameters of stavudine test formulation were obtained as follows: was  h, was  g/L, t1/2 was  h, and was ( ) g·h/L. Calculated with , the bioavailability of two formulations was 105.0%.