Table of Contents Author Guidelines Submit a Manuscript
Journal of Spectroscopy
Volume 2014, Article ID 751458, 9 pages
http://dx.doi.org/10.1155/2014/751458
Research Article

Effects of UV-B Radiation on Near-Infrared Spectroscopy and Identification of Puccinia striiformis f. sp. tritici

1College of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, China
2College of Information and Electrical Engineering, China Agricultural University, Beijing 100083, China

Received 12 November 2014; Accepted 10 December 2014; Published 29 December 2014

Academic Editor: Austin Nevin

Copyright © 2014 Pei Cheng et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Based on near-infrared spectra of three physiological races of Puccinia striiformis f. sp. tritici (i.e., CYR31, CYR32, and CYR33) irradiated under four UV-B intensities (i.e., 0, 150, 200, and 250 μw/cm2), the effects of UV-B radiation on near-infrared spectroscopy of the pathogen were investigated in spectral region 4000–10000 cm−1, and support vector machine models were built to identify UV-B radiation intensities and physiological races, respectively. The results showed that the spectral curves under UV-B radiation treatments exhibited great differences compared with the corresponding control treatment (0 μw/cm2) in the spectral regions 5300–5600 cm−1 and 7000–7400 cm−1 and that the absorbance values of all the three physiological races increased with the enhancement of UV-B radiation intensity. Based on near-infrared spectroscopy, different UV-B radiation intensities could be identified and different physiological races could be distinguished from each other with high accuracies. The results demonstrated the utility and stability of the proposed method to identify the physiological races.