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Journal of Spectroscopy
Volume 2014, Article ID 841450, 6 pages
Research Article

Observation of Climacteric-Like Behavior of Citrus Leaves Using Fluorescence Spectroscopy

1Instituto de Física de São Carlos, Universidade de São Paulo, 13566-590 São Carlos, SP, Brazil
2Centro de Engenharia, Modelagem e Ciências Sociais Aplicadas, Universidade Federal do ABC, 09210-170 Santo André, SP, Brazil
3Departamento de Fitopatologia e Nematologia, Universidade de São Paulo, 13418-900 Piracicaba, SP, Brazil

Received 15 April 2014; Accepted 23 May 2014; Published 11 June 2014

Academic Editor: Rodolphe Jaffiol

Copyright © 2014 Caio B. Wetterich et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Observation of climacteric-like behavior in citrus leaves depends on the detection of ethylene. However, such detection requires a gas chromatographer and complex sample preparation procedures. In this work, fluorescence spectroscopy was investigated as a diagnostic technique for climacteric-like behavior in citrus leaves. Our results indicate that the chlorophyll fluorescence presents a time evolution consistent with the ethylene evolution. Therefore, fluorescence spectroscopy may be used to observe the climacteric-like behavior in citrus leaves.