Spectroscopic Study of Cytosine Methylation Effect on Thermodynamics of DNA Duplex Containing CpG Motif

Figure 2

Typical results of singular values decomposition applied to the set of 40 temperature dependent Raman spectra of self-complementary DNA octamer (data for OctAT). The second spectral component () reflects the main spectral changes connected with the duplex melting and the related scores () were employed to determine the melting temperature by the least square fit. The result of the fit is shown in red.