Spectroscopic Study of Cytosine Methylation Effect on Thermodynamics of DNA Duplex Containing CpG Motif

Table 2

Thermodynamic characteristics of duplex formation and effect of the cytosine methylation.

Sequence	CAT(m)CGATG		CTT(m)CGAAG		CAA(m)CGTTG
Sample	OctAT	mOctAT	OctTT	mOctTT	OctAA	mOctAA
Δ (kJ·mol^−1)	−262 ± 12	−283 ± 14	−193 ± 8	−176 ± 7	−255 ± 12	−255 ± 13
ΔΔ (kJ·mol^−1)	−21 ± 18		17 ± 11		0 ± 17
Δ (J·mol^−1·K^−1)	−753 ± 37	−813 ± 44	−549 ± 26	−494 ± 23	−731 ± 36	−726 ± 41
ΔΔ (J·mol^−1·K^−1)	−60 ± 58		55 ± 35		5 ± 55
Δ (kJ·mol^−1)	−28.7 ± 0.4	−30.9 ± 0.5	−22.7 ± 0.3	−23.2 ± 0.3	−28.6 ± 0.4	−29.7 ± 0.5
ΔΔ (kJ·mol^−1)	−2.1 ± 0.7		−0.5 ± 0.4		−1.2 ± 0.6