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Journal of Spectroscopy
Volume 2016, Article ID 9580136, 9 pages
Research Article

Probing into the Interaction of Nicotine and Bovine Submaxillary Mucin: NMR, Fluorescence, and FTIR Approaches

1College of Chemical Engineering, Sichuan University, Chengdu, Sichuan 610065, China
2Technology Center, China Tobacco Yunnan Industrial Co., Ltd., Kunming, Yunnan 650204, China

Received 13 December 2015; Revised 16 January 2016; Accepted 28 January 2016

Academic Editor: Rizwan Hasan Khan

Copyright © 2016 Xiaoxiang Liao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Nicotine, the important component of cigarette products, may have an impact on the oral environment after inhalation. The research of interaction between nicotine and bovine submaxillary mucin (BSM) contributes to understand the binding mechanism of nicotine and BSM, and the effects of nicotine on the structure and function of the mucin. NMR data demonstrated that the interaction between nicotine and BSM did exist, and it was pyrrolidyl ring of nicotine playing the major role in the binding. The quenching mechanisms of nicotine and BSM in different pH were different: for acidic environment, the quenching was dynamic; while it became static in the alkaline circumstance. Synchronous fluorescence spectra indicated that nicotine had effect on the microenvironment of the Trp rather than Tyr residue. Meanwhile, the impact of nicotine on the conformation of BSM was also confirmed by 3D fluorescence and FTIR spectra.