Photophysical Properties of Fluorescent Probe Thioflavin T in Crowded Milieu

Figure 1

Structure of the fluorescent probe thioflavin T (ThT) and crowding agents. (a) ThT. The angle of internal rotation of benzothiazole and aminobenzene rings of ThT relative to each other is shown; (b) Dextran-70 (MW 70000 Da). It is shown that the straight chain of Dextran-70 consists of α-1,6 glycosidic linkages between glucose molecules, while branches begin from α-1,3 linkages; : number of units (glucose molecules) with only α-1,6 glycosidic linkages and : number of units with α-1,6 and α-1,3 linkages. (с) Polyethylene glycol (PEG). In the work we used PEG-400 and PEG-12000 with MW 400 Da () and MW 12000 Da (), respectively.

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