Photophysical Properties of Fluorescent Probe Thioflavin T in Crowded Milieu

Figure 4

Fluorescence anisotropy of ThT in aqueous solution, in aqueous-glycerol solutions, and in aqueous solutions in the presence of crowding agents. The excitation laser impulse profile , the experimental and best-fit calculated decay curves of the vertical (2,3) and horizontal (4,5) components of the fluorescence excited by vertically polarized laser impulses at 420 nm and the change in fluorescence anisotropy over time for ThT in aqueous solution (a), ThT in aqueous solution in the presence of PEG-400 in concentration 400 mg/mL (b), ThT in aqueous solution in the presence of 46% glycerol (c), and ThT in aqueous solution in the presence of 99% glycerol (d) are shown. is the number of registered impulses in the time interval normalized to unity in the maximum. The data presented on (a), (b), and (c) were obtained with the use of spectrometer FOG100 and the data presented on (d) were obtained with the use of spectrometer FluoTime 300.

(a)

(b)

(c)

(d)