Photophysical Properties of Fluorescent Probe Thioflavin T in Crowded Milieu

Table 1

Spectral properties of ThT in the presence of crowding agents.

Object	Binding mode	, nm	ε⋅10^−4, M^−1cm^−1	, nm
ThT in aqueous solution [21]	—	412	3.2	490	0.0001	1
ThT in aqueous solution in the presence of Dextran-70 (400 mg/mL)	—	417	3.2	494	0.0005	5
ThT in aqueous solution in the presence of PEG-400 (400 mg/mL)	—	421	3.6	495	0.0008	8
ThT in aqueous solution in the presence of PEG-12000 (400 mg/mL)	—	420	3.6	497	0.0007	7
ThT in aqueous-glycerol solution with glycerol content 99% [21]	—	424	3.6	497	0.066	6.6·10^3
ThT incorporated into insulin amyloid fibrils [34, 35]	1	449	7.9	488	0.72	7.2·10^4
	2	448	2.3		0.27	2.7·10^4
ThT incorporated into lysozyme amyloid fibrils [36, 37]	1	451	5.3	484	0.44	4.4·10^4
	2	449	6.2		0.0001	1
ThT incorporated into amyloid fibrils formed from Abeta-peptide (1–42) [35]	1	442	8.3	488	0.19	1.9·10^4
	2	437	1.8		0.02	2·10^3

For comparison the characteristics of ThT bound to amyloid fibrils are given.
: molar extinction coefficient, : fluorescence quantum yield, : fluorescence quantum yield normalized by the value in water (), and and : wavelengths of maximum of the absorption and fluorescence spectra, respectively. For ThT-amyloid fibrils interaction data shown for different binding modes (types of binding with significantly different binding parameters).