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Journal of Spectroscopy
Volume 2017, Article ID 4289830, 7 pages
Research Article

Using a Spectrofluorometer for Resonance Raman Spectra of Organic Molecules

1Department of Physics and Astronomy, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia
2Research Chair on Laser Diagnosis of Cancers, Department of Physics and Astronomy, College of Science, King Saud University, Riyadh 11451, Saudi Arabia

Correspondence should be addressed to Vadivel Masilamani; moc.liamg@321alisam

Received 8 October 2016; Accepted 5 January 2017; Published 21 March 2017

Academic Editor: Jau-Wern Chiou

Copyright © 2017 Vadivel Masilamani et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Scattering (Rayleigh and Raman) and fluorescence are two common light signals that frequently occur together, confusing the researchers and graduate students experimenting in molecular spectroscopy laboratories. This report is a brief study presenting a clear discrimination between the two signals mentioned, employing a common spectrofluorometer such as the PerkinElmer LS 55. Even better, the resonance Raman signal of a molecule (e.g., acetone) can be obtained elegantly using the same instrument.