Photo-Induced Cell Damage Analysis for Single- and Multifocus Coherent Anti-Stokes Raman Scattering Microscopy

Figure 4

Temporal behaviors of the normalized two-photon fluorescence values S_damage,TPF observed at the nucleus of HeLa cells. (a) Temporal behaviors of the normalized two-photon fluorescence values of the nucleus of individual cells with the single-focus excitation, the multifocus excitation, the control condition, and the fixed HeLa cell condition. Typical normalized two-photon fluorescence and white-light transmission images after starting of the laser irradiation of 0 and 20 min with (b) the single- and the (c) the multifocus excitations.