Photo-Induced Cell Damage Analysis for Single- and Multifocus Coherent Anti-Stokes Raman Scattering Microscopy

Figure 6

One-photon florescence analysis of photo-induced cell damage with DAPI fluorophores. Typical normalized one-photon fluorescence images after starting of the laser irradiation of 0, 20, and 40 min with (a) the single- and (b) the multifocus excitations. Dashed squares and dashed circles indicate the region of near-infrared light irradiation for the cell damage induction. Temporal behavior of one-photon fluorescence of DAPI fluorophores at the cell nucleus with (c) high and (d) low laser power irradiation conditions under the single-focus excitation (blue circle), the multifocus excitation (red square), and the control condition (green triangle). Error bars indicate standard deviation.

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