Simultaneous Determination of Four Compounds in a Nelumbo nucifera Seed Embryo by HPLC-DAD

Table 2

Intra- and interday precision of four compounds in the extract of lotus embryo by HPLC-DAD. Neferine (1), 1,2,3,4-tetrahydro-1-[(4-hydroxyphenyl) methyl]-2-methyl-7-isoquinolinol (2), 1-hydroxy-2-methylpropene (3), and 3-(prop-1-enyl)benzene-1,2,4,5-tetrol (4).

Compounds	Concentrations (μg/mL)	Intraday ()		Interday ()
		Mean (μg/mL)	RSD^a (%)	Mean (μg/mL)	RSD^a (%)
1	362.5	351.97	0.30	359.77	6.08
	181.25	225.79	0.53	222.35	0.87
	90.63	103.69	0.58	103.86	1.81
2	404.16	411.85	0.48	411.73	0.05
	202.08	205.06	0.56	205.02	0.34
	101.04	101.50	1.04	101.52	0.54
3	362.5	364.57	0.59	364.22	0.50
	181.25	183.53	1.35	181.32	0.90
	90.63	90.82	0.78	89.99	1.54
4	387.5	388.06	0.23	388.83	0.40
	193.75	193.93	0.44	193.49	0.26
	96.88	96.86	0.57	96.96	0.14

^aRSD: relative standard deviation.