Simultaneous Determination of Four Compounds in a Nelumbo nucifera Seed Embryo by HPLC-DAD

Table 3

Recoveries of 4 compounds in an N. nucifera embryo. Neferine (1), 1,2,3,4-tetrahydro-1-[(4-hydroxyphenyl) methyl]-2-methyl-7-isoquinolinol (2), 1-hydroxy-2-methylpropene (3), and 3-(prop-1-enyl)benzene-1,2,4,5-tetrol (4).

Compounds	Spiked amount (μg)	Measured amount (μg)	RSD (%)	Recovery^a (%)
1	180.18	181.25	1.74	99.41
	98.59	90.63	0.53	108.78
	49.06	45.31	1.12	108.27
2	194.54	202.08	1.03	96.27
	98.13	101.04	4.66	97.12
	50.93	50.52	1.55	100.81
3	180.15	181.25	0.65	99.39
	93.06	90.63	2.40	102.68
	45.70	45.31	2.12	100.86
4	199.36	193.75	0.78	102.90
	99.41	96.88	1.43	102.62
	48.89	48.44	0.17	100.93

^aRecovery (%) = (observed amount − original amount)/spiked amount × 100%.