Combined Raman Spectroscopy and Digital Holographic Microscopy for Sperm Cell Quality Analysis

Figure 5

Volume estimation procedure from the phase map. (a) Phase map. (b) Selected head region by Otsu’s threshold method. (c) Polygonal interpolation. (d) Final estimated sperm head volume. Scale bars: 5 μm [7].

(a)

(b)

(c)

(d)