Combined Raman Spectroscopy and Digital Holographic Microscopy for Sperm Cell Quality Analysis

Figure 7

(a) Average Raman spectra of 900 X- (purple line) and 900 Y- (blue line) sperm cell spectra acquired in the “fingerprint” spectral region. The experiment has been performed on three different bulls. (b) Measured peak area of the bands at 726 and 785 cm⁻¹, highlighting the different DNA contents measured for X- and Y-sperm cells; . (c) PCA score plots of PC2, PC3, and PC4 showing the separation of data corresponding to X- and Y-sperm cells. [6]. (d) Confusion matrix summarizing the correct and incorrect classifications for the analysed X- and Y-sperm spectra [6, 63, 64].

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