Combined Raman Spectroscopy and Digital Holographic Microscopy for Sperm Cell Quality Analysis

Figure 9

(a) Raman signal-to-noise ratio (SNR) measured as the ratio of the maximum Raman peak intensity at 1335 cm⁻¹ to the standard deviation of the region between 1750 and 1800 cm⁻¹ as function of the applied green light irradiation levels between 0 and 165 MJ/cm². (b) Representative spectra relative to the laser fluences of 0, 30, 61, and 107 MJ/cm² are shown. (c) Zoom of the spectra in the DNA and protein regions [8].

(a)

(b)

(c)