Stability-Indicating Photochemical Method for the Assay of Thiamine by Spectrophotometry

Table 2

Analytical parameters for the assay of thiochrome (TC).

λmax	370 nm
Linearity^a
Concentration range	0.20–2.00 mg%
Correlation coefficient	0.9999
Slope	654
SE of slope	0.0048
Intercept	0.0031
SE of intercept	0.00325
SD of intercept	0.0103
Recovery range (%)	99.3–100.7
Accuracy (%)^b ± SD	99.71 ± 0.50
Precision (% RSD)^c	0.50
LOD^d (mg)	0.052
LOQ^e (mg)	0.157

^aLinearity, . ^bAccuracy, at 100% concentration. ^cPrecision, at 100% concentration. ^dLimit of detection (LOD) = 3.3 × σ/S, where σ is the standard deviation and S is the slope of the standard curve. ^eLimit of quantitation (LOQ) = 10 × σ/S, where σ is the standard deviation and S is the slope of the standard curve.