Automation of the Peak Fitting Method in Bone FTIR Microspectroscopy Spectrum Analysis: Human and Mice Bone Study

Figure 5

Original spectrum in the amide area (dark blue line) recovered by the Gaussian sum fitting (dotted-black line) and the 8 individual peaks beneath (dashed and full lines) + 1 peak for the MMA (1730 cm⁻¹). The two peaks directly used to compute the Collagen Maturity parameter are the 1660 cm⁻¹ peak (dashed-purple line) and the 1690 cm⁻¹ peak (dashed-blue one).