Evaluation of Colorimetric BCA-Based Quantification of Hydrazide Groups on Magnetic Particles

Figure 2

The colorimetric determination of hydrazide groups using the BCA reagent. (a) Linear correlation of absorbance (570 nm) and ADH solution in the concentration range of 0.022–2.2 mmol/L (). (b) Standard curves obtained by the reaction of BCA with various amounts of hydrazide- and/or carboxyl-containing MPs (0-1.5 mg, ). (c) Standard curve obtained after the reaction of various amounts of hydrazide bead cellulose (1.2–120 μmol/L) with BCA (). (d) The comparison of absorbance from hydrazide bead cellulose (10 and 100 μL) and their equimolar amounts of standard ADH solutions (0.075 and 0.75 mmol).

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