Different Spectrophotometric Methods Manipulating Ratio Spectra Applied for the Analysis of Aclidinium in Duaklir^® Genuair^® Inhalation Powder

Table 3

Recovery study of aclidinium by adopting standard addition technique via the proposed ratio derivative and ratio subtraction methods.

Pharmaceutical conc. (µg/mL)	Pharmaceutical found (µg/mL)	Pure added (µg/mL)	% recovery
			Ratio derivative	Ratio subtraction
35	35.073^a	6	101.26	100.29
		8	100.23	101.28
	9.955^b
		10	98.25	99.67
Mean ± % RSD			99.91 ± 1.531	100.41 ± 0.809

^aAverage of five determinations by the ratio derivative method; ^baverage of five determinations by the ratio subtraction method.